Volume 11, Issue 1 (vol- 1 2005)                   Intern Med Today 2005, 11(1): 17-21 | Back to browse issues page

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Iraj Mansouri A, Noruzian E, Gholamhoseinian A. The relation between the copper level and ceruloplasmin in blood of the healthy people . Intern Med Today 2005; 11 (1) :17-21
URL: http://imtj.gmu.ac.ir/article-1-186-en.html
1- , mansori@icst.ac.ir
Abstract:   (13781 Views)
Background and Aim: Most of copper present in blood is incorporated in the structure of a protein called ceruloplasmin and only a small fraction of it is present as free copper. Undoubtedly, the separate determination of each of these forms needs a convenient, reliable, and accurate method of measurement. Therefore, an investigation on the precision and the accuracy of the method for determination of ceruloplasmin and the relationship between its concentration and the total copper level was studied Materials and Methods: In the present study, 90 serum samples from which 43 accessions belonged to men and 47 accessions belonged to women were collected from the laboratory of Kerman General Hospital, and their ceruloplosmin and total copper level were determined. Total copper levels were measured by flame atomic absorption spectroscopy (AAS), while the concentration of ceruloplasmin was determined by enzymatic oxidation of p-phenylenediamine method. Results: The results obtained indicate that the average levels of copper in the serum for men and women were 119±40µg/dl and 129±39µg/dl, and ceruloplasmin for them 36±11mg/dl and 37±11mg/dl, respectively. It should be mentioned that, the data, disregarding their sex, were found to be 124±38µg/dl and 36±11mg/dl respectively. The effect of temperature and storage on the level of ceruloplasmin was also studied. The results showed that keeping serum at room temperature for a long period does not significantly affect the ceruloplasmin level. The linear relationship between the concentration of ceruloplasmin and level of total copper was obtained as: Copper (µg/dl)=2.73+3.34 (mg/dl of ceruloplasmin). Conclusion: It seems that one could easily determine the ceruloplasmin concentration and then by referring to the above relationship calculate the otherwise hard to measure concentration of copper present in the serum. Also long time storage of the serum does not affect the Enzyme activity.
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Type of Study: Original | Subject: Internal Medicine
Received: 2008/05/19 | Published: 2005/04/15

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