Volume 23, Issue 1 (Winter 2017)                   Intern Med Today 2017, 23(1): 7-12 | Back to browse issues page

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1- Young Researchers and Elite Club, Felaverjan Branch, Islamic Azad University, Isfahan, Iran , e.shams88@yahoo.com
2- Microbiology Department, Tehran North Branch, Islamic Azad University, Tehran, Iran
3- “Toxicology Research Center” and “Toxicology Department, Pharmacy School”, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4- Biology Department, Felaverjan Branch, Islamic Azad University, Isfahan, Iran
5- Biophysics Department, of Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran
Abstract:   (5660 Views)

Aims: The increasing use of the electromagnetic devices in daily life leads to higher electromagnetic filed effects. The effects on the organic systems are contradictory and controversial. The aim of this study was to investigate the effects of different intensities and durations of the static magnetic fields on the living cells and their proliferation rate.

Materials & Methods: In the applied study, two HeLa cancer cell lines and human fibroblast natural cells were studied. At first, the cells were cultured on DMEN medium. Three magnetic intensities (7, 14, and 21T) and two durations (24 and 48h) were used, and the cells were treated by static magnetic field. The living cell percentage and cell proliferation rate were assessed by MTT method. Trypan blue was used in staining. And an optical microscope was used in enumeration. Data was analyzed by Graphpad Prism 5 using one-way ANOVA.  

Findings: The higher the static magnetic field and the more the duration were, the lesser the percentage of living cells and cell proliferation, showing a significant reduction in the HeLa cancer cells, while it was insignificant in the fibroblast natural cells. The highest reduction in the living cell percentage and cell proliferation rate was in 48-hour 21T (p<0.05).

Conclusion: The static magnetic field affects the HeLa cancer cells more than the fibroblast cells. The higher the field intensity and the more the duration are, the lesser the alive cell percentage and cell proliferation rate.

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Type of Study: Original | Subject: Physiology
Received: 2016/07/23 | Accepted: 2016/10/16 | Published: 2016/12/31

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