Aims: The present study intends to study the effect of volunteering exercises during adolescence on testosterone and corticosterone levels in male rats following childhood stress.

Materials &methods: In the present study, 36 male rats were selected as subjects and separated from their mothers from 2 to 14 days for 180 minutes. Then, to determine the experimental and control groups, on the 21st day, these randomly assigned random variables were divided into 3 groups. The groups included control, with stress separated from the mother, a wheel of two rodents. The first group experienced maternal separation from 2 to 14 days, and the control group was kept from the beginning with the mother. The training groups also started volunteering on the 21st birthday. The testosterone and cortisol levels of all groups were measured and the data were statistically analyzed by T and ANOVA methods at a significant level (P< 0.05).

Findings: The results showed that maternal stress severity significantly increased cortisol levels and decreased testosterone levels. On the other hand voluntary exercise, in comparison with the stress group, has increased testosterone levels and significantly reduced cortisol levels.

Conclusion: The results of the study showed that exercise, especially voluntary exercise, during adolescence, reduced stress and decreased depression and anxiety behaviors in adulthood.