Volume 26, Issue 2 (Spring 2020)
Intern Med Today 2020, 26(2): 142-155 |
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Aboeepoor S, Dehghani Ashkezari M, Aboee-Mehrizi F, Haghiralsadat B F, Nikoonahad Lotfabadi N. Designing and Characterizing Nano-carriers Containing Nepeta Persica Extract and Their Effect on Bone Cancer. Intern Med Today 2020; 26 (2) :142-155
URL: http://imtj.gmu.ac.ir/article-1-3312-en.html
URL: http://imtj.gmu.ac.ir/article-1-3312-en.html
Samanah Aboeepoor1 
, Mahmood Dehghani Ashkezari1 , Fateme Aboee-Mehrizi * 2, Bibi Fatemeh Haghiralsadat3 , Narges Nikoonahad Lotfabadi4
, Mahmood Dehghani Ashkezari1 , Fateme Aboee-Mehrizi * 2, Bibi Fatemeh Haghiralsadat3 , Narges Nikoonahad Lotfabadi4
1- Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran.
2- Department of Medicine, Yazd Branch, Islamic Azad University, Yazd, Iran. ,aboeef@yahoo.com
3- Medical Nanotechnology & Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Department of Biology, Faculty of Basic Sciences, Science and Arts University, Yazd, Iran.
2- Department of Medicine, Yazd Branch, Islamic Azad University, Yazd, Iran. ,
3- Medical Nanotechnology & Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Department of Biology, Faculty of Basic Sciences, Science and Arts University, Yazd, Iran.
Abstract: (2981 Views)
Aims Niosomes have been considered as carriers for targeted delivery of drugs in modern drug delivery systems. The Iranian Nepta (Nepta genus) has unique biological properties; thus, this plant was used in this study to prepare the optimized formulation of niosomes containing extract, and to evaluate its cytotoxicity.
Methods & Materials Initially, the extract of Iranian Nepta (N. persica) was prepared. Then, the nano-niosomal system, containing the extract was designed and synthesized using cholesterol and Span-60. The physicochemical properties of the system were evaluated by FTIR and SEM. MTT assay [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] was used to determine the toxicity of the Nepta extract and nano-system containing it against bone cancer cells (MG63).
Findings An optimized formulation was obtained using cholesterol and Span-60 with a ratio of 80:20. The amount of extract release from this formulation continued with a steady slope over a long period of time. The survival rate of MG63 cells against 100 μg/mL of the free form of N. persica extract and its niosomal form were 22% and 8.88%, respectively.
Conclusion The present research results suggested that N. persica extract exert anti-cancer effects and niosome could improve its anti-cancer efficiency. Therefore, it could be used as a proper carrier to deliver the extract to the target tissue.
Methods & Materials Initially, the extract of Iranian Nepta (N. persica) was prepared. Then, the nano-niosomal system, containing the extract was designed and synthesized using cholesterol and Span-60. The physicochemical properties of the system were evaluated by FTIR and SEM. MTT assay [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] was used to determine the toxicity of the Nepta extract and nano-system containing it against bone cancer cells (MG63).
Findings An optimized formulation was obtained using cholesterol and Span-60 with a ratio of 80:20. The amount of extract release from this formulation continued with a steady slope over a long period of time. The survival rate of MG63 cells against 100 μg/mL of the free form of N. persica extract and its niosomal form were 22% and 8.88%, respectively.
Conclusion The present research results suggested that N. persica extract exert anti-cancer effects and niosome could improve its anti-cancer efficiency. Therefore, it could be used as a proper carrier to deliver the extract to the target tissue.
Type of Study: Original |
Subject:
Basic Medical Science
Received: 2019/06/8 | Accepted: 2019/11/13 | Published: 2020/06/21
Received: 2019/06/8 | Accepted: 2019/11/13 | Published: 2020/06/21
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