Volume 27, Issue 4 (Autumn 2021)                   Intern Med Today 2021, 27(4): 550-565 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Gozali M, Ahmadizadeh C. Evaluation of Changes in NFKB Gene Expression Following Epstein-barr Virus and Its Participation in the Half-life of Patients With Acute Epstein-Barr Positive Lymphoblastic Leukemia. Intern Med Today 2021; 27 (4) :550-565
URL: http://imtj.gmu.ac.ir/article-1-3615-en.html
1- Department of Microbiology, Faculty of Basic Science, Ahar Branch, Islamic Azad University, Ahar, Iran.
2- Department of Microbiology, Faculty of Basic Science, Ahar Branch, Islamic Azad University, Ahar, Iran. , dr_ahmadizadeh@yahoo.com
Abstract:   (807 Views)
Aims: Leukemia is one of the most common childhood malignancies. The Epstein-Barr Virus (EBV) is a tumorigenic virus of the herpes family and causes a primary infection in young children. This study aimed to evaluate the increase in NFKB expression following the EBV virus and its contribution to the half-life of EBV-positive acute lymphoblastic leukemia patients.
Methods & Materials: In this case-control study, we examined blood samples of 60 patients referred to Tabriz Children’s Hospital for 6 months, Tabriz City, Iran, in 2019. RNA extraction was performed from the collected samples, and the quantity and quality of the extracted RNA were controlled by a NanoDrop device and gel electrophoresis. cDNA synthesis was performed from RNA extracted from the sample. NFKB gene expression was assessed using real-time PCR. The obtained data were analyzed using a t-test.
Findings: The results showed that the EBV virus decreased the expression of the NFKB gene in patients with acute lymphoblastic leukemia. The expression of the NFKB gene in patients with acute lymphoblastic leukemia increased significantly compared to the control group (P<0.05).
Conclusion: The NFKB gene can be used as a precursor for diagnosing acute lymphoblastic leukemia. However, further studies are required on EBV infection and acute lymphoblastic disease.
Full-Text [PDF 4950 kb]   (286 Downloads) |   |   Full-Text (HTML)  (535 Views)  
Type of Study: Original | Subject: Basic Medical Science
Received: 2020/11/21 | Accepted: 2021/02/27 | Published: 2021/10/1

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Internal Medicine Today

Designed & Developed by : Yektaweb