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Aims: Diabetic neourophaty is one of the long-term usual outcomes of diabetes. According to anti-tumor, anti-diabetic and anti-oxidant effects of Cannabis sativa, the aim of this research was to investigate the effect of Cannabis sativa alcoholic extract on Alpha motoneurons degeneration after sciatic nerve compression in diabetic rats.

Methods: This experimental laboratorial research was performed in 30 Wistar male rats with the weight of 300 to 350g in 5 “control”, “compression” , “compression+diabetes”, “compression+diabetes+treatment with 25mg/kg alcoholic extract of Cannabis sativa seed” and “compression+diabetes+treatment with 50mg/kg alcoholic extract of Cannabis sativa seed” groups. After preparing the alcoholic extract of Cannabis sativa seed, 18 rats were undergone diabetes induction and 24 rats were taken compression surgery. Spinal cord samples were taken from all 30 rats. Data was analyzed by Minitab 14 software and ANOVA test.

Results : Neuronal density of “compression” group (789 ± 28) was decreased significantly in comparison with “control” group (1766 ± 70). There was a significant difference between neuronal density of “compression” group and “compression+diabetes” (543 ± 14) group. Comparison of neuronal density between “compression+diabetes” group and rats treated with 25 and 50mg/kg doses of ethanolic extracts showed significant differences in both treated groups, the neuronal density was increased compare to “compression” and “compression+diabetic” groups.

Conclusion : Using alcoholic extract of Cannabis sativa as a neuroprotective agent can prevent the progression of neural system disorders as a result of hyperglycemia.

  

 

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Aims: Any damages to the nerve fiber blocking connection with the neurons cell body (vital cell substances synthesis part), can lead to degenerative changes in proximal and distal parts of the damaged neuron. Some plants due to the antioxidant substances are effective in the survival and division of the neurons. This study aimed to investigate the effect of alcoholic extract of Salvia chloroleuca leaves on destruction of spinal cord anterior horn alpha motoneurons after sciatic nerve injury in rats. Materials & Methods: In this experimental study 24 male Wistar rats aged 12weeks and weighting 200-250gr were provided. The animals were divided into 4 (each=6) control, damaged, damaged + treatment with 50mg/kg alcoholic extract and damaged+treatment with 75mg/kg alcohol extract groups. To calculate the alpha motoneurons in the anterior horn of the spinal cord in the right side the dissector method was used. Data were analyzed using Minitab 13 software by independent T and ANOVA tests. Findings: The mean density of alpha motoneurons in spinal anterior horn in control group was 1711.5±47.4, in damaged was 730.7±33.4, in damaged+treatment with 50mg/kg alcoholic extract was 1211.2±34.8 and in damaged+treatment with 75mg/kg alcoholic extract was 987.4±36.8. Mean difference between both treatment groups and damaged group was significant (p=0.001). Conclusion: Salvia chloroleuca leaf extract increases the density of alpha motor neurons in the anterior horn of the spinal cord in rats with sciatic nerve injury, and this increase in neuronal density is associated with the intake of the extract.