Showing 10 results for Abtahi
Dr. V. Omranifard, Dr. Ghr. Kheirabadi, Dr. Smm. Abtahi, Dr. M. Kamali,
Volume 13, Issue 2 (vol-2 2007)
Abstract
Background and Aim: This study was prompted by reports suggesting a high prevalence of unrecognized obsessive-compulsive disorder (OCD) in the dermatology clinics.
Materials and Methods:140 consecutive dermatology referrals were screened for DSM-IV OCD using the Yale-Brown Obsessive Compulsive Scale (YBOCS). Illness severity was rated on the Y-BOCS, and symptom profiles and dermatologic symptom were established for all cases.
Results: 31patients (22.1%) qualified for a DSM-IV diagnosis of OCD, all of them were previously undiagnosed. The range and type of OCD symptoms covered the normal clinical spectrum. 33(23.6%) 0f patients had more than 1 symptom, and among OCD symptoms over washing was the most common. 39.7% of the positive cases scored 16 or higher. Dermatologic symptoms were various and did not seem to bear a direct relationship with OCD.
Conclusion: These results suggest that there is a high prevalence of clinically relevant OCD in the dermatology clinics. This is an area that merits attention with regard to better recognition and treatment for OCD sufferers.
Seyyed Meysam Abtahi Froushani, Hadi Esmaili Gouvarchingaleh, Bahman Mansori Mothlag, Mohammad Babaei,
Volume 20, Issue 3 (Autumn 2014)
Abstract
Aims: Steroid hormones have powerful effect on all levels of the innate and adaptive immune systems. Among steroid hormones, estrogens play a key role in the regulation of homeostasis of bone marrow activity, cardiovascular system and central nervous system. The main purpose of this study was to determine the possible effects of moderating role of estrogen on humoral and cellular immune responses following the sheep red blood cells challenge in the mouse model.
Materials & Methods: 14 mice were divided into two groups and were immunized with SRBC antigen. The treated mice were injected with estrogen for two weeks (5mg/kg- intraperitoneally) from the beginning of the study. Specific humoral and cellular immunity, susceptibility of macrophages respiratory burst and proliferation of immune cells were measured. The Mann-Whitney test was used in order to compare using SPSS 19 software.
Findings: The 17 beta-estradiol treated mice showed a significant increase in DTH reaction. MTT test results showed an increase in the rate of lymphocyte proliferation in estrogen-treated group compared with control group. The spleen weight mean in treated mice group (28.62±1.76) showed a significant increase compared to control group (21.00±1.83) (p<0.01). The results of NBT reduction showed a decrease in spleen macrophages/monocyte respiratory burst capability.
Conclusion: It seems that 17 beta-estradiol can be considered as the moderating immune system compound.
Taheri Chadorneshin H., Afzalpour M.e., Abtahi H., Foadoddini M.,
Volume 20, Issue 4 (1-2015)
Abstract
Aims: Alpha tumor necrosis factor and Hydrogen peroxide increase in neurotrophins expression in several brain structures. The purpose of this study was to investigate the interactive effect of Hydrogen peroxide and neurotrophic tumor necrosis factor with brain-derived neurotrophic factor by glial cell-derived neurotrophic factor after severe exercise.
Materials & Methods: This experimental study was done on 16 adult Wistar albino rats 280g and 3months old. Animals were divided into two intense exercise and sedentary control groups. Animals ran for 6 weeks, 6 days a week, at the speed of 27m per minute and on treadmill for 60 minutes daily. Using kit, the content of BDNF, GDNF, and TNF-α were measured using sandwich ELISA and hydrogen peroxide levels was analyzed by colorimetric assay. Data analyzed by SPSS 16 and Independent-T test.
Findings: Hydrogen peroxide levels in the brain, in intense exercise group increased significantly compared with control group (p= 0.006). TNF-α, GDNF and BDNF Levels in the brain in intense exercise group significantly increased compared with control group (p=0.001).
Conclusion: Intense running on treadmill increase BDNF and GDNF content in brain of albino Wistar rats through increasing the H2O2 and TNF-α levels.
Mohammad Esmaei Afzalpour, Seyed Hosein Abtahi Eivari, Azam Rezazadeh, Amin Soluki,
Volume 21, Issue 2 (Summer 2015)
Abstract
Aims: Intense exercise damages tissues and disturb some cellular processes through oxidative stress and antioxidants can modulate intense exercise-induced oxidative stress. The aim of the present study was to examine the effect of Ziziphus jujube supplements on the glutathione peroxidase (GPX) and superoxide dismutase (SOD) activity in serum after a single session of resistance training. Materials & Methods: In this semi-experimental study, 24 young non-athletes females were purposefully selected and were randomly divided into two groups Ziziphus jujube consumption+intensive resistance exercise and intensive resistance exercise. The first group received 0.4g/kg of body weight of Ziziphus jujube daily for 3 weeks, but another group prohibited from Ziziphus jujube consumption. Both groups carried out a session of intensive resistance exercise consisting of 5 movements at 90% of one maximum repetition. Blood samples were measured in three phases baseline, after 3 weeks of the Ziziphus jujube consumption, and after the resistance exercise session. In order to valuation the enzymes activity the enzymatic method was used. Data were analyzed by the repeated measures ANOVA and LSD tests in SPSS 22 software. Findings: The Ziziphus jujube supplement significantly increased GPX activity (p=0.001) but it had no significant (p=0.19) influence on SOD activity. In addition, intensive resistance training significantly decreased the SOD (p=0.03) and GPX (p=0.02) activity immediately after exercise. Conclusion: Using Ziziphus jujube supplements improves the antioxidant enzyme activity of GPX, but this improvement is not likely enough to inhibit the depression of the antioxidant status after performing resistance exercise
M. Torkamani Noughabi , G.h. Vaezi, H. Abtahi Eivari, V. Hojati,
Volume 24, Issue 1 (Winter 2018)
Abstract
Aims: Yawning is a phylogenetic and contagious behavior that occurs with the stretching or penile erection in mammals, birds, and reptiles under different conditions. This study aimed at determining the acute and chronic effects of the injection of 1-(3-chlorophenyl) Piperazine and Mianserin on yawning and penile erection of adult male rats.
Materials and Methods: In this experimental study, 30 male Wistar rats (250-300g) were randomly divided to 3 groups (n=10), including the control group that received saline subcutaneous injection, the group that received 0.5mg/kg 1-(3-chlorophenyl) Piperazine subcutaneous injection, and the group that received 0.2mg/kg Mianserin subcutaneous injection. After the subcutaneous injections in acute phase (day1) and chronic phase (day14), the number of yawning and penile erection was observed for 60 minutes and it was recorded. The data were analyzed by SPSS 13 software using one-way analysis of variance, Tukey post-hoc, and paired t-test.
Findings: Compared with the control group, Mianserin group showed a significant increase in the number of yawning (p<0.01) and penile erection (p<0.05) in the acute phase. Compared with the acute phase, the chronic group of Mianserin showed a significant decrease in the number of yawning (p<0.01) and penile erection (p<0.05). Compared with the acute group, 1-(3-Chlorophenyl) Piperazine showed a significant increase in the number of yawning (p<0.05) only in the chronic phase.
Conclusion: The acute injection of Mianserin increases the number of yawning and penile erection of adult male rats and its chronic injection decreases them. 1-(3-Chlorophenyl) Piperazine increases the number of yawning only in the chronic phase.
M. Abdollahpourasl, Sh. Khezri, M. Abtahi Froushani, O. Cheraghi,
Volume 24, Issue 3 (Summer 2018)
Abstract
Aims: Hypiran is a commercial hydro-alcoholic extract of Hypericum perforatum. Its anti-inflammatory and immune modulatory benefits have been reported in several documents. This study was conducted to investigate the beneficial potential of Hypiran in the treatment of ameliorating experimental autoimmune encephalomyelitis (EAE).
Materials & Methods: In this experimental study, EAE was induced by guinea pig spinal cord homogenate and complete Freund’s adjuvant in 30 male Wistar rats (6-8 weeks old, weighing 100± 20 g). Hypiran administration (110 mg/kg-P.O.-daily) was initiated at day 12 post-immunization, when the rats developed a disability score. The brains and blood samples were collected on the day 36 and used for MDA, FRAP, NO and MPO experiments.
Findings: Hypiran-therapy led to a better situation in EAE rats. The lipid peroxidation level (MDA assay) was significantly increased in brain tissues of the EAE rat compared to that of the normal control one (P<0.001). Treatment with hypiran could significantly reduce the MDA levels in brain tissues of the EAE rats compared to that of the EAE rats without treatment. Moreover, Serum analysis showed that hypiran could significantly decline the nitric oxide levels as well as myeloperoxidase activity of the EAE rats compared to that of the EAE rats without treatment. Moreover, docking server analysis indicated that the hypiran could inhibit the MAO enzyme.
Conclusion: It seems that hypiran may be as a promising strategy to be treatment of Multiple Sclerosis patients.
Minoo Shaddel, Hamid Shamsi, Payman Tavakoli, Seyyed Meysam Abtahi Foroushani, Roya Shamsi,
Volume 25, Issue 3 (Summer 2019)
Abstract
Aims Aloe Vera is among the crucial medicinal plants, with proven anti-cancer effects. This study aimed to investigate the effect of plant extracts of Aloe Vera Leaf (AVL) on the proliferation of cell lines K562 (erythroleukemia) and Peripheral Blood Mononuclear Cells (PBMCs).
Methods & Materials The inner parts of the plant, including the gelatinous substance, were emptied, and the outer layer was used to prepare the lyophilized extract. The extract was dissolved in DMSO. K562 cells and PBMCs were cultured with 9.375, 18.75, 37.5, 75, 150, and 300 μg/mL concentrations of AVL for 24, 48, or 72 hours. After cultivation, the IC50 was determined for the K562 and normal PBMCs, using the MTT assay (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide). Doxorubicin was used as a positive control.
Findings The obtained data suggested that the viability of cells in both the leukemic cell line and normal PBMCs significantly decreased by the treatment with the extract in a dose-dependent manner; however, the AVL toxicity for PBMC was significantly more than K562. IC50 for the standard drug was also significantly less than AVL extract.
Conclusion AVL possesses cytotoxic effects for K562 and PBMCs. Nevertheless, it has no selective benefits and has more cytotoxic effects on PBMCs.
Yeganeh Feizi, Mohammad Esmaeil Afzalpur, Seyed-Hosein Abtahi-Eivary,
Volume 25, Issue 4 (Autumn 2019)
Abstract
Aims Physical activity is usually accompanied by free radicals’ production and oxidative stress. Moreover, to prevent adverse effects, coaches and athletes have to use proper supplementation. Therefore, the present study aimed to investigate the effect of short-term coenzyme Q10 supplementation on malondialdehyde and serum catalase enzyme activity following moderate and severe acute resistance training in inactive female students.
Methods & Materials In total, 27 female students were randomly divided into three groups; the groups were homogeneous and equal (two groups of resistance training and one control group). The experimental groups were subjected to moderate-intensity acute (70% 1RM) acute and severe acute activity (85% 1RM) and supplemented with coenzyme Q10 (30 mg /d). CAT and MDA were measured in ELISA using a human kit.
Findings Moderate and severe acute resistance activities did not alter MDA and catalytic activity (P>0.05); however, after 2 weeks of coenzyme Q10 supplementation, those resulted in a significant decrease in MDA (0.006 and 0.01, respectively) and CAT (0.04 and 0.007, respectively). There were no significant differences between the effects of two exercises (P>0.05).
Conclusion Short-term (two weeks) supplementation of coenzyme Q10 and severe acute resistance activity could reduce two important oxidative stress indexes (MDA and CAT).
Jafar Hajavi, Alireza Mohammadzadeh, Mojtaba Kianmehr, Abbasali Abbasnezhad, Mohadeseh Nasiri, Mahnaz Alie, Zohreh Pirzadeh Moghaddam, Zahra Elahi, Zahra Jamali, Seyyed Behnam Mazloum Shahri, Seyed Hossein Abtahi Eivary,
Volume 27, Issue 4 (Autumn 2021)
Abstract
Aims: Hepatitis B infection is one of the common diseases and the most prevalent communicable virus transferred by blood to the healthcare personnel. Active immunity, through vaccination, is the most effective way to prevent hepatitis B infection. The current study aimed to determine the protective antibody titer against HBs antigen in Gonabad University of Medical Sciences students, Gonabad City, Iran, 2018.
Methods & Materials: The present cross-sectional study was conducted on 416 students of Gonabad University of Medical Sciences. The HBsAb level was determined in blood samples by an Enzyme-Linked Immunosorbent Assay (ELISA) kit made in Iran. Data were analyzed using descriptive statistics like frequency distribution tables and inferential statistics as independent 2-sample t-test and Chi-square test to determine the relationship between variables. The significance level was considered P<0.05.
Findings: HBsAb level of 217 cases (51.8%) was below 10 IU/mL, 96 (61.3%) had an HBsAb level between 10 and 100 IU/mL, and in 106 (36.5%) cases, HBsAb level was above 100 IU/mL. There was no statistically significant difference between the means of HBsAb in terms of gender, age, and body mass index (P<0.05).
Conclusion: In general, more than 50% of the study participants had mild immunity against hepatitis B viruses. Therefore, in these people, it is recommended to check the antibody titer periodically to ensure immunity against hepatitis B.
Maryam Talebileili, Nowruz Delirezh, Seyed Meysam Abtahi Foroushani,
Volume 29, Issue 1 (Winter 2022)
Abstract
Aims The present study aimed to assess the effects of spleen-cell-conditioned medium treated with different concentrations of adenosine on the 4T1 breast cancer cell line.
Materials & Methods For this purpose, mouse spleens were isolated in completely sterile conditions, and splenocytes were then isolated for culture. Different concentrations of adenosine (25, 50, and 100 μM doses), phytohemagglutinin solution, and culture medium containing Fetal Bovine Serum (FBS) were added to splenocytes and incubated for 72 h. Thereafter, splenocytes were incubated by adding fresh culture medium without serum for 24 h. This fluid was collected and stored in a freezer at -80 for further assessment. The conditioned medium was mixed with an equal volume of the culture medium containing 4T1 cancer. The studied groups included control containing untreated 4T1 cancer cells, spleen-cell-conditioned medium untreated with adenosine, spleen-cell-conditioned medium treated with adenosine ∼25 μM, spleen-cell-conditioned medium treated with adenosine ∼5 μM, and the fifth group: spleen-cell-conditioned medium treated with adenosine ∼10 μM. After 48 h, the viability of 4T1 cells was measured by MTT and NR tests, and the amount of apoptosis in the cells was evaluated by vital dye staining.
Findings The spleen-cell-conditioned medium is able to damage cancer cells and causes a significant reduction in neutral red uptake or damage to the cancer cell membrane. In the MTT test, a decrease was observed in the mitochondrial power; that is to say, a decrease in cell viability of cancer cells compared to the control group. In the apoptosis test, a percentage of cancer cells changed color from green to red. This function of the conditioned medium was strengthened by adding adenosine ∼25 μM to the spleen cell conditioned medium so that the amount of neutral red uptake was markedly reduced and caused a further decrease in the mitochondrial power of cancer cells. It also increased apoptosis in cancer cells so that most cancer cells changed color to red. The beneficial effect of the spleen-cell-conditioned medium decreased by increasing the concentration of adenosine to 5 µM so that there was no statistically significant difference with the untreated spleen-cell-conditioned medium. When we increased the concentration of adenosine to 10 µM, the killing efficacy disappeared, and there was no significant difference with the control group.
Conclusion Based on the results, the spleen-cell-conditioned medium was able to kill a percentage of cancer cells. When we treat the spleen-cell-conditioned medium with low concentrations of adenosine, we observe a marked improvement in the killing efficacy of cancer cells, as well as an interaction between immune cells and cancer cells. Nonetheless, when the concentration of adenosine increases, the killing efficacy of cancer cells is lost and completely reversed, resulting in the growth of cancer cells.
