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Aims: The increasing use of the electromagnetic devices in daily life leads to higher electromagnetic filed effects. The effects on the organic systems are contradictory and controversial. The aim of this study was to investigate the effects of different intensities and durations of the static magnetic fields on the living cells and their proliferation rate.

Materials & Methods: In the applied study, two HeLa cancer cell lines and human fibroblast natural cells were studied. At first, the cells were cultured on DMEN medium. Three magnetic intensities (7, 14, and 21T) and two durations (24 and 48h) were used, and the cells were treated by static magnetic field. The living cell percentage and cell proliferation rate were assessed by MTT method. Trypan blue was used in staining. And an optical microscope was used in enumeration. Data was analyzed by Graphpad Prism 5 using one-way ANOVA.  

Findings: The higher the static magnetic field and the more the duration were, the lesser the percentage of living cells and cell proliferation, showing a significant reduction in the HeLa cancer cells, while it was insignificant in the fibroblast natural cells. The highest reduction in the living cell percentage and cell proliferation rate was in 48-hour 21T (p<0.05).

Conclusion: The static magnetic field affects the HeLa cancer cells more than the fibroblast cells. The higher the field intensity and the more the duration are, the lesser the alive cell percentage and cell proliferation rate.

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Aims Because cancer is the leading cause of death worldwide, finding a better way to treat it seems essential. Doxorubicin is one of the most common drugs in the treatment of cancer, which has many negative and toxic effects. Therefore, efforts to produce effective anticancer drugs through screening natural compounds, such as animal toxins continue. This study aimed to evaluate the effect of Naja naja oxiana snake venom in comparison with doxorubicin on the proliferation rate and concentration of malondialdehyde in the human cervical cancer cell line (HeLa) and fibroblast cells (HFF).
Methods & Materials HeLa and normal fibroblast cancer cell lines were exposed to different concentrations of snake venom and doxorubicin for 24 and 48 hours. The amplification rate was determined using trypan blue staining and malondialdehyde (MDA) concentration was measured to evaluate the effects of oxidative stress. Data were analyzed using SPSS software, version 19.
Findings The results showed that with increasing concentration and treatment time with snake venom and doxorubicin, the cell proliferation rate decreases, and MDA content increases. The highest decrease in proliferation rate and increase in MDA concentration were observed in the HeLa cancer cell line treated with 500 µg/mL of snake venom for 48 hours.
Conclusion In comparison with doxorubicin, snake venom has a significant inhibitory effect on the HeLa cancer cell line with minimal effect on normal fibroblast cells.