Lobat Jafarzadeh, Roya Habibian, Mahmood Rafieian Kopaei , Zohre Mohammadzade,
Volume 21, Issue 3 (Autumn 2015)
Abstract
Aims: Kelussia odoratissima is a plant from Umbelliferae family that has nutritional and pharmacological properties. The antispasmodic effect of this plant has been mentioned in Iranian traditional medicine. The aim of study was to assess the effect of alcoholic extract of Kelussia odoratissima on uterus contractions of adult rats. Materials & Methods: In this experimental study, 16 adult female Wistar rats were studied and were randomly divided into two groups (each consisted of 8 rats) receiver of Tyrod solution (first group) and receiver of Dejalon solution (second group) groups. A 10-15mm sample of uterus smooth muscle were placed in an organ bath containing Tyrod or Dejalon solution (10ml), and contractions were recorded with Isometric method after inducing with KCl and Oxytocin. The 0.125, 0.25 and 0.5mg/ml final concentrations of the alcoholic extract of Kelussia odoratissima was added to the organ bath. Data were statistically analyzed by SPSS 17 software using repeated measures ANOVA. Findings: The alcoholic extract of Kelussia odoratissima significantly decreased the KCl-induced (p<0.001) and Oxytocin-induced (p<0.002) contractions in a concentration dependent manner. Conclusion: The alcoholic extract of Kelussia odoratissima inhibits the contractions of adult rat uterus in a concentration dependent manner. Keywords Kelussia odoratissima [Not in MeSH] Uterine Contraction [http://www.ncbi.nlm.nih.gov/mesh/68014590] Rats [http://www.ncbi.nlm.nih.gov/mesh/68051381] Potassium Chloride [http://www.ncbi.nlm.nih.gov/mesh/68011189] Oxytocin [http://www.ncbi.nlm.nih.gov/mesh/68010121]
Mrs Faeze Tehranian, Mr Hossein Nezami, Mr Mohammad Hossein Jafarzadeh Maivan, Mr Javad Akbarzadeh Sani, Mr Jafar Hajavi,
Volume 28, Issue 4 (Autumn 2022)
Abstract
Aims Chemokine receptor type 5 is recognized as the main co-receptor in HIV infection. Many studies have shown that the deletion of 32-base pairs (bp) of both alleles of CCR5 leads to the reduction or elimination of the expression of this gene in immune cells and the natural resistance to HIV infection. This study aims to determine the frequency of delta 32 mutations in healthy people, referring to the health center of Gonabad City, Iran, to focus on finding new treatment methods for the treatment of HIV.
Methods & Materials In this descriptive cross-sectional study, after obtaining ethical approval and informed consent, blood samples were collected from 293 healthy people along with ethylenediaminetetraacetic acid anticoagulant. Genomic DNA extraction was carried out by the kit, and the investigation of delta 32 (∆32) mutation of the CCR5 gene was investigated via the polymerase chain reaction method.
Findings Our results showed that out of 293 people who referred to the health center, 269 people (91.8%) had a healthy gene without mutation (188 bp), 15 people (5.1%) had heterozygous mutations (188 bp and 156 bp), and 9 people (3.07%) had a homozygous mutation on both alleles of CCR5 (156 bp).
Conclusion Based on the results, the homozygous mutation rate of Gonabad Region, Iran, in the studied community is higher compared to similar studies, and more epidemiological and molecular studies are needed for better conclusions.
