S.d. Sadoughi,
Volume 23, Issue 4 (9-2017)
Abstract
Aims: Silver nanoparticles, through free radical production, can cause oxidative stress. The purpose of this study was to determine the effect of crocin on Bax/Bcl-2 ratio, lipid peroxidation and antioxidant enzymes activity in liver tissue of chick embryo treated with silver nanoparticles.
Materials & Methods: In this experimental study, 45 Ross 308 Fertilized chicken eggs were randomly divided into five groups (control and experimental groups). On day 10 of incubation, the control group received 0.5 ml of saline solution in an amniotic sac of embryos and experimental groups 1, 2, 3 and 4 were treated with one injection of 0.5 ml of silver nanoparticle 200 ppm and a size of 60 nm. On day 12 of incubation, the experimental groups 2, 3 and 4 were treated with crocin 0.5 mg/ml in concentrations of 100, 200 and 300 μg/ml. On day 20 of incubation, levels of Bax, Bcl-2, malondialdehyde (MDA) and antioxidant enzymes of the liver tissue were measured. Data were analyzed by SPSS 20 software, using one-way ANOVA and Tukey's post hoc test.
Findings: In the group of silver nanoparticles compared to the control group, the levels of Bcl-2 and antioxidant enzymes decreased and Bax and malondialdehyde levels increased. In groups of silver nanoparticles with concentrations of 100, 200 and 300 μg/ml of crocin compared to the group of silver nanoparticles alone, the levels of Bcl-2 and antioxidant enzymes increased in dose-dependent manner, and Bax and malondialdehyde levels decreased in dose-dependent manner (p<0.05).
Conclusion: Dose-dependent injection of crocin decreases oxidative stress, lipid peroxidation and apoptosis in liver tissue of chick embryo by decrease of toxicity of silver nanoparticles.
M. Abdollahpourasl, Sh. Khezri, M. Abtahi Froushani, O. Cheraghi,
Volume 24, Issue 3 (7-2018)
Abstract
Aims: Hypiran is a commercial hydro-alcoholic extract of Hypericum perforatum. Its anti-inflammatory and immune modulatory benefits have been reported in several documents. This study was conducted to investigate the beneficial potential of Hypiran in the treatment of ameliorating experimental autoimmune encephalomyelitis (EAE).
Materials & Methods: In this experimental study, EAE was induced by guinea pig spinal cord homogenate and complete Freund’s adjuvant in 30 male Wistar rats (6-8 weeks old, weighing 100± 20 g). Hypiran administration (110 mg/kg-P.O.-daily) was initiated at day 12 post-immunization, when the rats developed a disability score. The brains and blood samples were collected on the day 36 and used for MDA, FRAP, NO and MPO experiments.
Findings: Hypiran-therapy led to a better situation in EAE rats. The lipid peroxidation level (MDA assay) was significantly increased in brain tissues of the EAE rat compared to that of the normal control one (P<0.001). Treatment with hypiran could significantly reduce the MDA levels in brain tissues of the EAE rats compared to that of the EAE rats without treatment. Moreover, Serum analysis showed that hypiran could significantly decline the nitric oxide levels as well as myeloperoxidase activity of the EAE rats compared to that of the EAE rats without treatment. Moreover, docking server analysis indicated that the hypiran could inhibit the MAO enzyme.
Conclusion: It seems that hypiran may be as a promising strategy to be treatment of Multiple Sclerosis patients.
