Showing 5 results for نانوذرات
S.d. Sadoughi,
Volume 23, Issue 4 (9-2017)
Abstract
Aims: Silver nanoparticles, through free radical production, can cause oxidative stress. The purpose of this study was to determine the effect of crocin on Bax/Bcl-2 ratio, lipid peroxidation and antioxidant enzymes activity in liver tissue of chick embryo treated with silver nanoparticles.
Materials & Methods: In this experimental study, 45 Ross 308 Fertilized chicken eggs were randomly divided into five groups (control and experimental groups). On day 10 of incubation, the control group received 0.5 ml of saline solution in an amniotic sac of embryos and experimental groups 1, 2, 3 and 4 were treated with one injection of 0.5 ml of silver nanoparticle 200 ppm and a size of 60 nm. On day 12 of incubation, the experimental groups 2, 3 and 4 were treated with crocin 0.5 mg/ml in concentrations of 100, 200 and 300 μg/ml. On day 20 of incubation, levels of Bax, Bcl-2, malondialdehyde (MDA) and antioxidant enzymes of the liver tissue were measured. Data were analyzed by SPSS 20 software, using one-way ANOVA and Tukey's post hoc test.
Findings: In the group of silver nanoparticles compared to the control group, the levels of Bcl-2 and antioxidant enzymes decreased and Bax and malondialdehyde levels increased. In groups of silver nanoparticles with concentrations of 100, 200 and 300 μg/ml of crocin compared to the group of silver nanoparticles alone, the levels of Bcl-2 and antioxidant enzymes increased in dose-dependent manner, and Bax and malondialdehyde levels decreased in dose-dependent manner (p<0.05).
Conclusion: Dose-dependent injection of crocin decreases oxidative stress, lipid peroxidation and apoptosis in liver tissue of chick embryo by decrease of toxicity of silver nanoparticles.
Forogh Javaheri Houshi, Asieh Abbassi-Daloii, Ahmad Abdi, Seyed Javad Ziaolhagh,
Volume 25, Issue 3 (7-2019)
Abstract
Aims Silver nanoparticles are among the most valuable products of nanoscale technology, widely used in various sciences. The present study investigated the effects of biochemical silver nanoparticles on the structure of the heart tissue of non-observatory rats in the course of aerobic training.
Methods & Materials In this experimental study, 30 male Wistar rats aged 8 to 12 weeks and weighing 34.9±202 g were studied. The rats were randomly divided into 6 groups of control, aerobic training, aerobic training, and nanobiological injection, aerobic and-nanochemical injection, nanobiological injection, and nanochemical injection. Chemical and biological silver was injected intraperitoneally after a period of aerobic training. The specimens were discarded after 48 hours, and the heart tissue was removed.
Findings The obtained results revealed the tissue changes, including irregularities and the convergence of chemical nanosilver group significantly increased, compared to the controls. Additionally, in the biological group, there was a slight dispersion of blood in some areas. Following the aerobic training and injection of toxic nanosilver, there was no irregularities, detachment, and hypertension. Only in some areas, sporadically, the accumulation of blood cells was observed in the aerobic training and nanochemical groups.
Conclusion More tissue damage occurred in chemical silver nanoparticles, than the biological nanoparticle. Possibly, aerobic training can be highly predictive of these effects.
Asadollah Asadi, Arash Abdolmaleki,
Volume 25, Issue 4 (9-2019)
Abstract
Aims Nanoparticles (particles with a diameter of 10-500 nm) are currently used in the cosmetics industry as well as for pharmaceuticals, diagnostic imaging, and tissue engineering. Since these nanoparticles are used in industry and drug delivery, they can also be used by pregnant women. Thus, the current study investigated the teratogenic and cytotoxic effects of Zinc Sulfide (ZnS) nanoparticles on the embryo and their fibroblastic cell culture.
Methods & Materials Zinc sulfide (ZnS) nanoparticles were synthesized. Then, nanoparticles at the concentrations of 5, 10, 15, 30, and 40 mg/mL/egg were injected into the air sac of the eggs in three replicates on the third day of incubation. Next, the treated and control eggs, on day 19 of incubation were opened, and embryos were weighted, and the relevant mortality rate was recorded. Fibroblast cells were isolated, cultured, and treated from the control embryo, and morphological changes and cell survival percentages were recorded.
Findings The obtained results revealed that the embryos’ survival rate depends on the nanoparticle concentration. As a result, at the highest concentration, only 36.32% of the embryos survived, and the lethal dose 50% (LD50) was equal to 32.47 mg/egg. Morphological study of the treated embryos club foot and skeletal staining suggested the deletion of caudal vertebrate. The cytotoxicity study results of ZnS nanoparticles on fibroblastic cells indicated the survival fractions of 88.45%, 68.75%, and 49.32%, respectively, and its IC50 value was measured aas1460 μM.
Conclusion The present study results suggested that ZnS nanoparticles had no significant toxic effects on the embryos and culture of chicken fibroblastic cells at low concentrations.
Elaheh Moradi, Mobina Ahmadi, Narges Tavakoli, Fateme Nasirzadeh, Nastaran Yoosofnejad, Jalal Mardaneh,
Volume 28, Issue 2 (3-2022)
Abstract
Aims In recent years, the synthesis of nanoparticles (Ag-NPs) by plants has been done on a larger scale because it is faster and cheaper. There is also an increase in pathogenicity caused by antibiotic-resistant Staphylococcus aureus. In this study, the biosynthesis of silver nanoparticles by using an extract of mountain pistachio (Pistaciaatlantica) and its antimicrobial properties have been reported.
Methods & Materials The extract of Pistaciaatlantica as the reducing agent was used for the biosynthesis of silver nanoparticles. The formation of nanoparticles was confirmed using a spectrophotometer (UV). The resulting nanoparticles were analyzed to determine the Z-Average (d.nm) and the dispersion index (PDI) of the nanoparticles using dynamic light scattering (DLS) and its inhibitory activity and lethality (MBC) against Staphylococcus aureus (S. aureus) were investigated. The diameter of the aura of bacterial growth was also measured.
Findings The UV-Vis spectrum shows an absorption band of about 350-450 nm, which represents the biological Ag nanoparticles. Size and morphological properties of nanoparticles were performed by DLS which show that hydrodynamic diameter (Z-Average) is 1132 nm and PDI number is 0.373, indicating a uniform particle size distribution and nanoparticle stability. The inhibitory and lethal properties of Pistaciaatlantica nanoparticles on S. aureus species (PTCC 1764) are 12.5 micrograms per milliliter. Also, the diameter of the halo of bacterial growth is 12 mm which was observed at a concentration of 1000 μg/mL.
Conclusion Pistaciaatlantica bio-silver nanoparticles had good antimicrobial activity against S. aureus
Masoud Rezaei,
Volume 28, Issue 3 (6-2022)
Abstract
Aims In recent years, iron oxide nanoparticles have shown incredible possibilities in biomedical applications due to their non-toxic function in biological systems. Furthermore, these nanoparticles have multifunctional applications, such as antibacterial, antifungal, and anticancer effects in medicine due to their magnetic properties.
Methods & Materials In this article, 49 articles related to iron oxide nanoparticles and their wide applications in medicine and biotechnology were collected from 1998 to 2022 through article search databases from Google Scholar, Pubmed, and Scopus.
Findings Iron oxide nanoparticles are not only used as antibacterial, antifungal, and anticancer agents but also they can be functionalized with different molecules and used to treat and diagnose cancer. Due to the adverse effects of some of these molecules, phytochemicals of plant extracts are used today as an alternative to functionalize these nanoparticles. The main advantage of iron oxide nanoparticles is the appropriate bio-distribution in the biological sample compared to other drug delivery systems. The magnetic properties of iron oxide nanoparticles in hyperthermia and contrast media in magnetic resonance imaging have attracted the attention of many researchers.
Conclusion Iron oxide nanoparticles can be effective in targeted treatment and diagnosis in biomedicine. To improve anticancer and antimicrobial therapies, researchers have used these nanoparticles with different approaches. Considering the wide applications and results of iron oxide nanoparticles in medicine, this review article has been written to discuss the methods of synthesis of iron oxide nanoparticles, the types of coatings used to functionalize them, and the different applications in cancer treatment and diagnosis.
