fa اثر درمانی محیط کشت‌ حاصل از سلول‌های بنیادی‌ مزانشیمی مشتق از بافت چربی بر تغییرات هیستومورفومتریک بیضه موش در مدل آزواسپرمی القایی با بوسولفان فيزيولوژی پزشکی Physiology پژوهشی Original <p dir="RTL" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:justify;"><strong><span style="font-family:iransharp_printingsize,sans-serif;">اهداف: </span></strong><span style="font-family:iransharp_printingsize,sans-serif;">محیط کشت</span> <span style="font-family:iransharp_printingsize,sans-serif;">رویی</span> <span style="font-family:iransharp_printingsize,sans-serif;">سلول</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">های</span> <span style="font-family:iransharp_printingsize,sans-serif;">بنیادی</span> <span style="font-family:iransharp_printingsize,sans-serif;">مزانشیمی</span> <span style="font-family:iransharp_printingsize,sans-serif;">به</span><span style="font-family:iransharp_printingsize,sans-serif;">‌</span><span style="font-family:iransharp_printingsize,sans-serif;">دلیل</span> <span style="font-family:iransharp_printingsize,sans-serif;">دارابودن</span> <span style="font-family:iransharp_printingsize,sans-serif;">متابولیت</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">ها،</span> <span style="font-family:iransharp_printingsize,sans-serif;">فاکتورهای</span> <span style="font-family:iransharp_printingsize,sans-serif;">رشد</span> <span style="font-family:iransharp_printingsize,sans-serif;">و</span> <span style="font-family:iransharp_printingsize,sans-serif;">سیتوکین</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">ها</span> <span style="font-family:iransharp_printingsize,sans-serif;">تاثیر</span> <span style="font-family:iransharp_printingsize,sans-serif;">مثبتی</span> <span style="font-family:iransharp_printingsize,sans-serif;">در</span> <span style="font-family:iransharp_printingsize,sans-serif;">ترمیم</span> <span style="font-family:iransharp_printingsize,sans-serif;">بافت</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">های</span> <span style="font-family:iransharp_printingsize,sans-serif;">آسیب</span><span style="font-family:iransharp_printingsize,sans-serif;">‌</span><span style="font-family:iransharp_printingsize,sans-serif;">دیده</span><span style="font-family:iransharp_printingsize,sans-serif;"> دارد. هدف مطالعه </span><span style="font-family:iransharp_printingsize,sans-serif;">حاضر،</span> <span style="font-family:iransharp_printingsize,sans-serif;">بررسی</span><span style="font-family:iransharp_printingsize,sans-serif;"> اثر درمانی محیط کشت</span><span style="font-family:arial,sans-serif;">&rlm;</span> <span style="font-family:iransharp_printingsize,sans-serif;">حاصل</span> <span style="font-family:iransharp_printingsize,sans-serif;">از</span> <span style="font-family:iransharp_printingsize,sans-serif;">سلول</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">های</span> <span style="font-family:iransharp_printingsize,sans-serif;">بنیادی</span> <span style="font-family:iransharp_printingsize,sans-serif;">مزانشیمی</span><span style="font-family:arial,sans-serif;">&rlm;</span> <span style="font-family:iransharp_printingsize,sans-serif;">مشتق</span> <span style="font-family:iransharp_printingsize,sans-serif;">از</span> <span style="font-family:iransharp_printingsize,sans-serif;">بافت</span> <span style="font-family:iransharp_printingsize,sans-serif;">چربی</span> <span style="font-family:iransharp_printingsize,sans-serif;">بر تغییرات هیستومورفومتریک بیضه موش تحت تیمار با بوسولفان بود.</span></p> <p dir="RTL" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:justify;"><strong><span style="font-family:iransharp_printingsize,sans-serif;">مواد و روش</span></strong><strong><span style="font-family:arial,sans-serif;">&rlm;</span></strong><strong><span style="font-family:iransharp_printingsize,sans-serif;">ها</span></strong>: <span style="font-family:iransharp_printingsize,sans-serif;">در این مطالعه تجربی، 18 سر موش سوری نر بالغ به‌طور تصادفی به سه گروه کنترل (بدون تیمار)، شاهد آزواسپرمی و تجربی تقسیم شدند. گروه‌های شاهد و تجربی با دو تزریق درون‌صفاقی بوسولفان با دوز 10میلی‌گرم بر کیلوگرم به‌فاصله 21 روز دچار آزواسپرمی شدند. گروه تجربی، 35 روز پس از تزریق دوم بوسولفان، </span><span style="font-family:iransharp_printingsize,sans-serif;">تحت درمان با محیط کشت</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;"> رویی سلول</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">های بنیادی مزانشیمی </span><span style="font-family:arial,sans-serif;">&rlm;&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">مشتق از بافت چربی قرار گرفت و پس از 60 روز از تزریق محیط کشت</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;"> رویی، بیضه موش‌ها جدا </span><span style="font-family:iransharp_printingsize,sans-serif;">و</span> <span style="font-family:iransharp_printingsize,sans-serif;">بررسی</span> <span style="font-family:iransharp_printingsize,sans-serif;">هیستومورفومتریک </span><span style="font-family:iransharp_printingsize,sans-serif;">انجام شد. </span><span style="font-family:iransharp_printingsize,sans-serif;">نتایج توسط نرم‌افزار </span><span dir="LTR"><span style="font-family:cambria,serif;"><span style="font-size:10.0pt;">SPSS 18</span></span></span><span style="font-family:iransharp_printingsize,sans-serif;"> و به‌کمک آزمون</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">های</span><span style="font-family:iransharp_printingsize,sans-serif;"> آنالیز واریانس یک‌طرفه، تعقیبی توکی</span>&nbsp;<span style="font-family:iransharp_printingsize,sans-serif;">و من‌ویتنی تحلیل شدند.</span></p> <p dir="RTL" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:justify;"><strong><span style="font-family:iransharp_printingsize,sans-serif;">یافته</span></strong><strong><span style="font-family:arial,sans-serif;">&rlm;</span></strong><strong><span style="font-family:iransharp_printingsize,sans-serif;">ها</span></strong><strong><span style="font-family:iransharp_printingsize,sans-serif;">:</span></strong> <span style="font-family:iransharp_printingsize,sans-serif;">قطر</span> <span style="font-family:iransharp_printingsize,sans-serif;">و</span> <span style="font-family:iransharp_printingsize,sans-serif;">مساحت</span> <span style="font-family:iransharp_printingsize,sans-serif;">لومنی</span> <span style="font-family:iransharp_printingsize,sans-serif;">لوله</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">های</span> <span style="font-family:iransharp_printingsize,sans-serif;">منی</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">ساز</span> <span style="font-family:iransharp_printingsize,sans-serif;">در</span> <span style="font-family:iransharp_printingsize,sans-serif;">سه</span> <span style="font-family:iransharp_printingsize,sans-serif;">گروه</span> <span style="font-family:iransharp_printingsize,sans-serif;">اختلاف</span> <span style="font-family:iransharp_printingsize,sans-serif;">معنی</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">داری</span> <span style="font-family:iransharp_printingsize,sans-serif;">با</span> <span style="font-family:iransharp_printingsize,sans-serif;">یکدیگر</span> <span style="font-family:iransharp_printingsize,sans-serif;">نداشتند،</span><span style="font-family:iransharp_printingsize,sans-serif;"> اما </span><span style="font-family:iransharp_printingsize,sans-serif;">در</span> <span style="font-family:iransharp_printingsize,sans-serif;">گروه</span> <span style="font-family:iransharp_printingsize,sans-serif;">تجربی</span><span style="font-family:iransharp_printingsize,sans-serif;"> قطر کلی، ضخامت و مساحت سلولی، مساحت مقطع عرضی و شاخص اسپرماتوژنز لوله</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">های</span> <span style="font-family:iransharp_printingsize,sans-serif;">منی</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">ساز</span><span style="font-family:iransharp_printingsize,sans-serif;"> نسبت به گروه کنترل کاهش معنی‌دار &nbsp;(05/0</span><span dir="LTR"><span style="font-family:cambria,serif;"><span style="font-size:10.0pt;">p</span></span></span><span dir="LTR"><span style="font-family:cambria,serif;"><</span></span><span style="font-family:iransharp_printingsize,sans-serif;">)و نسبت به گروه شاهد آزواسپرمی افزایش معنی‌دار نشان دادند &nbsp;(001/0</span><span dir="LTR"><span style="font-family:cambria,serif;"><span style="font-size:10.0pt;">p</span></span></span><span dir="LTR"><span style="font-family:cambria,serif;"><</span></span><span style="font-family:iransharp_printingsize,sans-serif;">). همچنین تعداد </span><span style="font-family:iransharp_printingsize,sans-serif;">و</span> <span style="font-family:iransharp_printingsize,sans-serif;">تراکم</span> <span style="font-family:iransharp_printingsize,sans-serif;">عددی</span> <span style="font-family:iransharp_printingsize,sans-serif;">لوله</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">های</span> <span style="font-family:iransharp_printingsize,sans-serif;">منی</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">ساز</span> <span style="font-family:iransharp_printingsize,sans-serif;">در</span> <span style="font-family:iransharp_printingsize,sans-serif;">گروه</span><span style="font-family:iransharp_printingsize,sans-serif;"> شاهد آزواسپرمی</span><span style="font-family:arial,sans-serif;">&rlm;</span> <span style="font-family:iransharp_printingsize,sans-serif;">نسبت به گروه‌های کنترل و تجربی افزایش معنی</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">داری</span> <span style="font-family:iransharp_printingsize,sans-serif;">داشت</span><span style="font-family:iransharp_printingsize,sans-serif;"> (001/0</span><span dir="LTR"><span style="font-family:cambria,serif;"><span style="font-size:10.0pt;">p<</span></span></span><span style="font-family:iransharp_printingsize,sans-serif;">).</span></p> <p dir="RTL" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:justify;"><strong><span style="font-family:iransharp_printingsize,sans-serif;">نتیجه</span></strong><strong><span style="font-family:arial,sans-serif;">&rlm;</span></strong><strong><span style="font-family:iransharp_printingsize,sans-serif;">گیری</span></strong>: <span style="font-family:iransharp_printingsize,sans-serif;">فاکتورهای</span> <span style="font-family:iransharp_printingsize,sans-serif;">ترشحی</span> <span style="font-family:iransharp_printingsize,sans-serif;">محیط</span> <span style="font-family:iransharp_printingsize,sans-serif;">کشت</span> <span style="font-family:iransharp_printingsize,sans-serif;">رویی</span> <span style="font-family:iransharp_printingsize,sans-serif;">حاصل</span> <span style="font-family:iransharp_printingsize,sans-serif;">از</span> <span style="font-family:iransharp_printingsize,sans-serif;">سلول</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">های</span> <span style="font-family:iransharp_printingsize,sans-serif;">بنیادی</span> <span style="font-family:iransharp_printingsize,sans-serif;">بافت</span> <span style="font-family:iransharp_printingsize,sans-serif;">چربی</span> <span style="font-family:iransharp_printingsize,sans-serif;">می</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">تواند</span> <span style="font-family:iransharp_printingsize,sans-serif;">در</span><span style="font-family:iransharp_printingsize,sans-serif;"> ترمیم اسپرماتوژنز در موش</span><span style="font-family:arial,sans-serif;">&rlm;</span><span style="font-family:iransharp_printingsize,sans-serif;">های</span> <span style="font-family:iransharp_printingsize,sans-serif;">نابارورشده</span> <span style="font-family:iransharp_printingsize,sans-serif;">با</span> <span style="font-family:iransharp_printingsize,sans-serif;">بوسولفان</span> <span style="font-family:iransharp_printingsize,sans-serif;">موثر</span><span style="font-family:iransharp_printingsize,sans-serif;"> باشد.</span></p> <p><strong>Aims:</strong> Conditioned medium of mesenchymal stem cells have a positive effect on repairing damaged tissues because of having metabolites, growth factors and cytokines. The aim of current study was to investigate the healing effect of conditioned medium of adipose tissue-derived stem cells on histomorphometric changes of testis in treated mice with busulfan.</p> <p><strong>Materials & Methods</strong><strong>:</strong> In this experimental study, 18 adult male mice were divided into the three equal groups including control group (Without treatment), azoospermia sham and experimental groups. The sham and experimental groups were induced azoospermia by two injection of 10 mg/kg busulfan intraperitoneally, with 21 days interval. 35 days after the second injection of busulfan, the experimental group treated with conditioned medium of adipose tissue-derived stem cells, and 60 days after conditioned medium injection, the testes of mice were removed and histomorphometric evaluation was done. The results were analyzed by SPSS 18 software using Mann-Whitney, ANOVA and Tukey&#39;s post hoc-test.</p> <p><strong>Findings: </strong>The lumen diameter and area of the seminiferous tubules in the three groups did not have any significant difference; however, the total diameter, cellular diameter, cellular area, cross-sectional area and spermatogenesis index of the seminiferous tubules were significantly decreased in the experimental group compared to the control group (p˂0.05), while they were significantly increased compared to the azoospermia sham group (p˂0.001). Also, the number of tubules and numerical density of the seminiferous tubules were significantly increased in the azoospermia sham compared to the control and experimental groups (p˂0.001).</p> <p><strong>Conclusion:</strong> Secretory factors of conditioned medium from adipose tissue-derived stem cells could be effective in the restoration of spermatogenesis in busulfan-induced infertile mice.</p> <p></p> آزواسپرمی, بافت چربی, موش, محیط کشت رویی, سلول بنیادی مزانشیمی Azoospermia, Adipose Tissue , Mice, Culture Media, Conditioned , Mesenchymal Stromal Cells 235 240 http://imtj.gmu.ac.ir/browse.php?a_code=A-10-1428-11&slc_lang=fa&sid=1 A. Payehdar آرش پایه دار ebrahim.hossini@yahoo.com 8000319475328460024332 8000319475328460024332 No “Biology Department, Science Faculty, Shiraz Branch” and “Biology Department, Science Faculty, Fars Science and Research Branch”, Islamic Azad University, Shiraz, Iran گروه زیست شناسی، دانشکده علوم، واحد شیراز، دانشگاه آزاد اسلامی،شیراز،ایران. E. Hosseini ابراهیم حسینی ebrahim.hossini@yahoo.com 8000319475328460024333 8000319475328460024333 Yes “Biology Department, Science Faculty, Shiraz Branch” and “Biology Department, Science Faculty, Marvdasht Branch”, Islamic Azad University, Shiraz, Iran D. Mehrabani داود مهربانی ebrahim.hossini@yahoo.com 8000319475328460024334 8000319475328460024334 No Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran مرکز تحقیقات فناوری ترانسژنیک و سلول‌های بنیادی، دانشگاه علوم پزشکی شیراز، شیراز، ایران M. Forouzanfar محسن فروزانفر ebrahim.hossini@yahoo.com 8000319475328460024335 8000319475328460024335 No Biology Department, Science Faculty, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran گروه زیست‌شناسی، دانشکده علوم، واحد مرودشت، دانشگاه آزاد اسلامی، مرودشت، ایران