Volume 28, Issue 2 (Spring 2022)                   Intern Med Today 2022, 28(2): 244-263 | Back to browse issues page

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1- Department of Agricultural Engineering, Quchan Branch, Islamic Azad University, Quchan, Razavi Khorasan Province, Iran.
2- Student in the field of experimental Sciences, Shahed Reyhaneh Al-Nabi High School in Gonabad , Gonabad, Razavi Khorasan Province, Iran.
3- Department of Microbiology, Infectious Diseases Research Center, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Razavi Khorasan Province, Iran. , Jalalmardaneh@yahoo.com
Abstract:   (524 Views)
Aims In recent years, the synthesis of nanoparticles (Ag-NPs) by plants has been done on a larger scale because it is faster and cheaper. There is also an increase in pathogenicity caused by antibiotic-resistant Staphylococcus aureus. In this study, the biosynthesis of silver nanoparticles by using an extract of mountain pistachio (Pistaciaatlantica) and its antimicrobial properties have been reported.
Methods & Materials The extract of Pistaciaatlantica as the reducing agent was used for the biosynthesis of silver nanoparticles. The formation of nanoparticles was confirmed using a spectrophotometer (UV). The resulting nanoparticles were analyzed to determine the Z-Average (d.nm) and the dispersion index (PDI) of the nanoparticles using dynamic light scattering (DLS) and its inhibitory activity and lethality (MBC) against Staphylococcus aureus (S. aureus) were investigated. The diameter of the aura of bacterial growth was also measured.
Findings The UV-Vis spectrum shows an absorption band of about 350-450 nm, which represents the biological Ag nanoparticles. Size and morphological properties of nanoparticles were performed by DLS which show that hydrodynamic diameter (Z-Average) is 1132 nm and PDI number is 0.373, indicating a uniform particle size distribution and nanoparticle stability. The inhibitory and lethal properties of Pistaciaatlantica nanoparticles on S. aureus species (PTCC 1764) are 12.5 micrograms per milliliter. Also, the diameter of the halo of bacterial growth is 12 mm which was observed at a concentration of 1000 μg/mL.
Conclusion Pistaciaatlantica bio-silver nanoparticles had good antimicrobial activity against S. aureus
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Type of Study: Original | Subject: Laboratory Medicine
Received: 2021/05/24 | Accepted: 2021/09/18 | Published: 2022/03/1

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