logo
Volume 18, Issue 3 (Autumn 2012)                   Intern Med Today 2012, 18(3): 87-94 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ahmadianpour M R, Abdolmaleki P, Moula S J, Hosseinkhani S. Effect of static magnetic field on apoptosis induction and altering the cell cycle of T-lymphoblastoid cells. Intern Med Today 2012; 18 (3) :87-94
URL: http://imtj.gmu.ac.ir/article-1-1400-en.html
1- Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
2- Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran , parviz@modares.ac.ir
3- Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
4- Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:   (8161 Views)

 Aims: Apoptosis or programmed cell death is a normal physiological process of cell that leads to active and natural development and maintenance of tissue homeostasis. Apoptosis occurs due to different external factors. This study aimed to investigate a low risk method to induce apoptosis in human leukemia cells using static magnetic field (SMF).

 Methods: In this laboratory research, the cultured human T-lymphoblastoid cell line (Jurkat E6.1), exposed to 6mT SMF for 6 and 24 hours. Luminometry, flowcytometry and western blot were used to measure total apoptosis rate, primary apoptosis rate and phosphorylated ATM and E2F1 proteins, respectively.

 Results: Increasing in the rate of apoptosis in treated cells according to control cells was significant 36 hours after treatment with 6mT SMF for 6 (p=0.011) and 24 (p=0.055) hours. 36 hours after treatment with 6mT SMF, the first significant difference was seen between treated and control cells (p<0.001). 24 hours after cells exposure to 6mT SMF, cells population was decreased in G2 and S and 36 hours after treatment, the population was increased in G2 and S and decreased in G1. Exposure to 6mT SMF increased the amount of phosphorylated ATM protein in 1981 Serin and E2F1 protein in 31 Serin positions.

 Conclusion: 6mT SMF induces apoptosis in exposed Jurkat cells by enhancing and activating ATM and E2F1 proteins.

 

Full-Text [PDF 742 kb]   (3047 Downloads)    
Type of Study: Original | Subject: Basic Medical Science
Received: 2012/01/2 | Accepted: 2013/05/5 | Published: 2013/05/5

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.