Volume 18, Issue 1 (2012; Vol. 18, No. 2 2012)                   Intern Med Today 2012, 18(1): 26-36 | Back to browse issues page

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Ebrahimzadeh Bideskan A, Nikmard F, Mohammadzadeh E, Razavi H, Fazel A. Lectinhistochemistry Study of Glycoconjugate Terminal Sugars During Rat kidney Development . Intern Med Today 2012; 18 (1) :26-36
URL: http://imtj.gmu.ac.ir/article-1-1502-en.html
1- , ebrahimzadehba@mums.ac.ir
Abstract:   (7944 Views)
Abstract Background and Aim: Developmental processes are mediated by cell surface and extracellular matrix (ECM) interactions that are regulated by the molecular mechanism. The aim of this study was to determine Glycoconjugates terminal sugars distribution and the changes during kidney development in rats using Lectinhistochemical technique. Materials and Methods: Thirty Wistar Rat embryos at different stages from days 14 (E14) to 18 days of gestation were fixed in formalin, embedded in paraffin and cut into 5µm thickness sections serially. The sections were incubated with different HRP-lectins from Lotus tetragonolobus (LTA), Maclura pomifera (MPA) and Ulex europeus agglutinine (UEA-I). Staining intensity was determined by means of blind method and the sections were graded to compare different embryonic stages. Results: Our findings showed that the reactions of MPA with podocyts started at E14 and increased afterwards (p<0.05). Mesenchymal cells reacted with MPA on E14, E 16 and disappeared at E18 (p<0.05). Collecting duct reaction with MPA was moderate on E 16. Mesenchymal cells reaction with UEA-I was moderate on E14 to E16 but decreased on E18 (p<0.05). A weak reaction was observed in collecting duct with UEA-I on E16. Proximal and distal tubules as well as collecting ducts showed a weak reaction with LTA in E18. No reaction was observed in Podocytes. Mesenchymal cells reacted on E14 but did not react on E16 and E18 with UEA-I lectin. Conclusion: According to the results, it is concluded that distributions and changes of glycoconjugates with terminal sugars L-Fuc (α2-4) GlcNAc, Gal (β1-3) GalNAc and L-fuc (α 1→3) Gal (β 1-4) Glc, are regulated during rat kidney development.
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Type of Study: Applicable | Subject: Laboratory Medicine
Received: 2012/05/4 | Published: 2012/04/15

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