Volume 20, Issue 1 (4-2014)                   Intern Med Today 2014, 20(1): 1-6 | Back to browse issues page

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Behroozi J, Divsalar A. Comparison the effect of Bee venom and Aspirin on the fructation of human hemoglobin. Intern Med Today 2014; 20 (1) :1-6
URL: http://imtj.gmu.ac.ir/article-1-1850-en.html
1- Kharazmi university
2- Kharazmi university , javad_behroozi65@yahoo.com
Abstract:   (6113 Views)
Aims: Fructation causes structural changes in the proteins which finally changes or destroys the protein's function. The aim of this study was to investigate the anti-fructation effect of honey bee venom and compare it with aspirin. Methods: Hemoglobin extracted from healthy and nonsmoker subjects and its concentration was determined using optical- UV spectrometry. The bovine serum albumin (BSA) was used as the standard protein. In order to evaluate the effect of honey bee venom and aspirin, hemoglobin in the presence of these two substances was also fructuated. The release of heme group from protein and the changes in hemoglobin soret band was done by optical-UV spectrometry. The amount of free amines available in hemoglobin during fructation in the presence of aspirin and honey bee venom was measured by changes in the fluorescence florscasmine emission method. To investigate the structural changes of fructated hemoglobin protein spectropolaimetry and circular bicolor spectrophotometery method were used. Data were analyzed using InStat 3 software and One-way ANOVA test. Results: Hemoglobin incubation in the presence of fructose decreased the absorption of soret band of fructated hemoglobin compared to control. The amount of free amine in the presence of honey bee venom in of 20 and 40μg/ml had no significant difference with free amine in the presence of aspirin. Honey bee venom inhibited the change in second structure of hemoglobin dose-dependently during fructation. Conclusion: Honey bee venom has relatively similar effect with aspirin to inhibit hemoglobin fructation process.
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Type of Study: Original | Subject: Basic Medical Science
Received: 2013/09/29 | Accepted: 2014/03/19 | Published: 2014/03/19

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