Volume 27, Issue 1 (Winter 2020)
Intern Med Today 2020, 27(1): 82-97 |
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Hassanpour Soleimani S, Abbassi Daloii A, Abdi A, Zilaei Bori S. Effect of a 6-week Aerobic Exercise Program on High-mobility Group Box 1 Gene Expression in Aortic Tissue of Diabetic Rats. Intern Med Today 2020; 27 (1) :82-97
URL: http://imtj.gmu.ac.ir/article-1-3530-en.html
URL: http://imtj.gmu.ac.ir/article-1-3530-en.html
1- Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.
2- Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran. ,abbasi.daloii@gmail.com
3- Department of Physical Education & Sport Sciences, Masjed-Soliman Branch, Islamic Azad University, Masjed-Soleiman, Iran.
2- Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran. ,
3- Department of Physical Education & Sport Sciences, Masjed-Soliman Branch, Islamic Azad University, Masjed-Soleiman, Iran.
Abstract: (2718 Views)
Aims: Type 2 diabetes is a rising problem and a significant risk factor for small and large vessel disease. The present study aims to investigate the effect of a 6-week aerobic exercise program on High-mobility Group Box 1 (HMGB1) gene expression in aortic tissue of diabetic rats.
Methods & Materials: In this experimental study, 40 male rats aged 8 weeks were randomly selected. Once familiarized with the exercise protocol, they were divided into four groups of healthy-control (n=10), diabetic-control (n=10), healthy-exercise (n=10), and diabetic-exercise (n=10). Type 2 diabetes was first induced, and then the rats run on a treadmill for 6 weeks, 5 sessions per week. After 12-14 h of fasting and 72 h after the last session, aortic tissue sampling was performed for HMGB1 analysis by quantitative real-time polymerase chain reaction (q-RT PCR) technique. Data analysis was performed using 1-way ANOVA and Tukey post hoc test at the significant level of P<0.05.
Findings: Induction of type 2 diabetes led to a significant increase in HMGB1 gene expression in rats (P=0.001), significantly reduced following aerobic exercise (P=0.003). Aerobic exercise also reduced the expression of the HMGB1 gene in healthy rats (P=0.000).
Conclusion: Aerobic exercise may have a protective effect by reducing the HMGB1 gene expression in the aortic tissue of diabetic rats. It can be used as an effective non-pharmacological method to improve diabetes-induced inflammation and prevent vascular disorders.
Methods & Materials: In this experimental study, 40 male rats aged 8 weeks were randomly selected. Once familiarized with the exercise protocol, they were divided into four groups of healthy-control (n=10), diabetic-control (n=10), healthy-exercise (n=10), and diabetic-exercise (n=10). Type 2 diabetes was first induced, and then the rats run on a treadmill for 6 weeks, 5 sessions per week. After 12-14 h of fasting and 72 h after the last session, aortic tissue sampling was performed for HMGB1 analysis by quantitative real-time polymerase chain reaction (q-RT PCR) technique. Data analysis was performed using 1-way ANOVA and Tukey post hoc test at the significant level of P<0.05.
Findings: Induction of type 2 diabetes led to a significant increase in HMGB1 gene expression in rats (P=0.001), significantly reduced following aerobic exercise (P=0.003). Aerobic exercise also reduced the expression of the HMGB1 gene in healthy rats (P=0.000).
Conclusion: Aerobic exercise may have a protective effect by reducing the HMGB1 gene expression in the aortic tissue of diabetic rats. It can be used as an effective non-pharmacological method to improve diabetes-induced inflammation and prevent vascular disorders.
Type of Study: Original |
Subject:
Physiology
Received: 2020/05/10 | Accepted: 2020/08/12 | Published: 2021/01/1
Received: 2020/05/10 | Accepted: 2020/08/12 | Published: 2021/01/1
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