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Background and Aim: In most infections and diseases, to cure and decrease the effect of disease, induction and increasing power of immuno system are necessary. Nigella sativa is a plant that has been used for treatment of many diseases. In this survey, the effect of Nigella sativa is surveyed in strengthening of Monocyte phagocytosis so that the use and mechanism of this plant for strengthening the phagocytosis of main cells of immune system are characterized. Materials and Methods: For this purpose, 21 male quinea pigs were selected and divided into three groups: control group given nothing, treatment group with taking 6.25% Nigella sativa nutrient and another treatment group with taking 12.5% Nigella sativa nutrient for 3 weeks. At an even period, blood samples were taken and peripheral blood mononuclear cells (PBMC) were separated from each subject's Heparin Blood in the Faikol-Hypak style. Finally, the three tests, that is, reduction Nitro Blue Tetrazolium (NBT) renewal, phagocytosis with yeast and latex bead floressance were given. Results: The results have shown that increasing of phagocytosis in Monocyte is meaningful (P≤0.05). Conclusion: Using Black seed with food causes the increasing of phagocytosis in mononuclear cells and increasing of phagocytosis activity for uptake of foreign particles and pathogens. Thus, this substance can be used to define pathogens through activating Monocyte-Macrophage pathway, that is, the connector between the innate and adaptive immuno systems

Keywords: Nigella sativa, PBMC, phagocytosis

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