Volume 18, Issue 3 (Autumn 2012)                   Intern Med Today 2012, 18(3): 87-94 | Back to browse issues page

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1- Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
2- Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran , parviz@modares.ac.ir
3- Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
4- Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:   (9026 Views)

 Aims: Apoptosis or programmed cell death is a normal physiological process of cell that leads to active and natural development and maintenance of tissue homeostasis. Apoptosis occurs due to different external factors. This study aimed to investigate a low risk method to induce apoptosis in human leukemia cells using static magnetic field (SMF).

 Methods: In this laboratory research, the cultured human T-lymphoblastoid cell line (Jurkat E6.1), exposed to 6mT SMF for 6 and 24 hours. Luminometry, flowcytometry and western blot were used to measure total apoptosis rate, primary apoptosis rate and phosphorylated ATM and E2F1 proteins, respectively.

 Results: Increasing in the rate of apoptosis in treated cells according to control cells was significant 36 hours after treatment with 6mT SMF for 6 (p=0.011) and 24 (p=0.055) hours. 36 hours after treatment with 6mT SMF, the first significant difference was seen between treated and control cells (p<0.001). 24 hours after cells exposure to 6mT SMF, cells population was decreased in G2 and S and 36 hours after treatment, the population was increased in G2 and S and decreased in G1. Exposure to 6mT SMF increased the amount of phosphorylated ATM protein in 1981 Serin and E2F1 protein in 31 Serin positions.

 Conclusion: 6mT SMF induces apoptosis in exposed Jurkat cells by enhancing and activating ATM and E2F1 proteins.

 

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Type of Study: Original | Subject: Basic Medical Science
Received: 2012/01/2 | Accepted: 2013/05/5 | Published: 2013/05/5

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