Volume 22, Issue 1 (Winter 2016)                   Intern Med Today 2016, 22(1): 77-88 | Back to browse issues page

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Noori-Daloii M, Faraji K. High Resolution Melt Analysis (HRM) and its Strategic Applications Especially in Molecular Genetics. Intern Med Today 2016; 22 (1) :77-88
URL: http://imtj.gmu.ac.ir/article-1-2134-en.html
1- tehran universit of medical sciences , nooridaloii@sina.tums.ac.ir
2- tehran universit of medical sciences
Abstract:   (7539 Views)

Introduction: In wide and developing worlds of cellular and particularly molecular sciences, the most important issue that leads to paying more attention to and use a technique, is its critical features; high sensivity, specificity, velocity and also being available and cheap. High Resolution Melting (HRM), may be one of these techniques. Its usage is growing among others significantly in investigations, for its special advantages. HRM is based on the pattern and characteristic of DNA when melting. DNA melting temperature (Tm), is the temperature that 50% of DNA molecules have become single stranded and 50% are double stranded. The pattern of getting single stranded is specific and unique for every DNA strand; and differences are shown as a graph called “melting curve". These are effective on DNA Tm: the value of bases G and C in both DNA strands (CG%), the length of fragment, the static effects of bases on each strand and the hetrozygocity. In fact, the differences among melting curves results in recognition the special fragment. The way of being single stranded can be followed by fleurcence colors which attached to double stranded DNAs.

Conclusion: High-resolution melting is powerful, fast, comprehensive and useful technique for using in molecular laboratory that can be considered as simple and fast approach for genotyping, mutation detection, matching sequence and methylation study.

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Type of Study: Review | Subject: Basic Medical Science
Received: 2015/01/14 | Accepted: 2015/07/7 | Published: 2015/08/19

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